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KMID : 0613820190290060662
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Journal of Life Science
2019 Volume.29 No. 6 p.662 ~ p.670
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Effect of Graviola Leaves Extracts on Antioxidant Activity and Melanin Production
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Lee Kyu-Won
Jang Ji-Won
Park Su-Min
Kang Ki-Hun
Yoon Hye-Soo
Ha Ye-Jin
Jeon So-Jeong
Ko Hye-Ju
Kim Moon-Moo
Oh Yung-Hee
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Abstract
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The purpose of this study was to investigate the effect of methanolic extracts of graviola, Annona muricate leaves (AMME) on antioxidant activity and melanin production. First of all, DPPH radical and reducing power were performed to determine the antioxidant effect of AMME and organic solvent fractions. AMME and organic solvent fractions showed antioxidative activity in a concentration dependent manner. The ethyl acetate fraction of AMME among organic solvent fractions showed the highest antioxidant activity. Moreover, tyrosinase activity was performed to confirm the effect of organic fractions on melanin production. AMME, ethyl acetate, and hexane fractions increased tyrosinase activity a dose dependent manner. Next, the hexane fraction with the best effect on melanin synthesis in AMME organic solvent fraction was divided into 12 fractions by silica column chromatography. Among them, the fraction 7 and 8 showed the highest DPPH radical scavenging activity and reducing power. In addition, the fraction 7 and 8 at 64 μg/ml showed melanin synthesis by 260% and 184%, respectively. Finally, the fraction 8 at 4 μg/ml showed melanin synthesis by 34% in B16F1 cells. LC-MS analysis showed that fraction 7 and fraction 8 have a molecular weight of 617 and 619, respectively. FT-IR analysis showed that fractions 7 and 8 is similar to bis(2-hydroxyethly)dimerate. Above results suggest that graviola leaves extracts could be applicable to the development of natural antioxidants or hair cosmetics which are related to the promoting effect of melanin production.
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KEYWORD
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Annona muricate, B61F1, graviola, melanin synthesis, tyrosinase
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